plx 311 krab dcas9 (Addgene inc)
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plx 311 krab dcas9
Plx 311 Krab Dcas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plx+311+krab+dcas9/pLX_311-KRAB-dCas9+(Plasmid+%2396918)/bio_rxiv__64898__2026__03__13__711679-66-12-13
Average 93 stars, based on 28 article reviews
Plx 311 Krab Dcas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plx+311+krab+dcas9/pLX_311-KRAB-dCas9+(Plasmid+%2396918)/bio_rxiv__64898__2026__03__13__711679-66-12-13
Average 93 stars, based on 28 article reviews
plx 311 krab dcas9 - by Bioz Stars,
2026-09
93/100 stars
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Construct:Article Title: The human Y and inactive X chromosomes similarly modulate autosomal gene expression. Article Snippet: We obtained the following constructs from Addgene for use in our experiments: 1) for CRISPRi, pLX_311-KRAB-dCas9 (Addgene: #96918), a KRAB-dCas9 blasticidin-selectable lentiviral expression vector38; 2) sgOpti (#85681), a puromycin-selectable structurally optimized guide RNA (gRNA) lentiviral expression vector67,68; 3) psPAX2 (#12260), a second generation lentiviral packaging plasmid; and 4) pCMV-VSV-G (#8454), a lentiviral envelope protein plasmid.69 We purified all plasmids using the EndoFree Maxi Kit (Qiagen). e8 Cell Genomics 4, 100462, January 10, 2024 Weused the top five guide sequences for ZFX and ZFY from the humanCRISPRi v2 gRNA library.50We tested these guides for ZFX or ZFY knockdown and chose the two guides that gave the most robust response for the final experiments. .. We obtained the following constructs from Addgene for use in our experiments: 1) for CRISPRi, Expressing:Article Title: The human Y and inactive X chromosomes similarly modulate autosomal gene expression. Article Snippet: We obtained the following constructs from Addgene for use in our experiments: 1) for CRISPRi, pLX_311-KRAB-dCas9 (Addgene: #96918), a KRAB-dCas9 blasticidin-selectable lentiviral expression vector38; 2) sgOpti (#85681), a puromycin-selectable structurally optimized guide RNA (gRNA) lentiviral expression vector67,68; 3) psPAX2 (#12260), a second generation lentiviral packaging plasmid; and 4) pCMV-VSV-G (#8454), a lentiviral envelope protein plasmid.69 We purified all plasmids using the EndoFree Maxi Kit (Qiagen). e8 Cell Genomics 4, 100462, January 10, 2024 Weused the top five guide sequences for ZFX and ZFY from the humanCRISPRi v2 gRNA library.50We tested these guides for ZFX or ZFY knockdown and chose the two guides that gave the most robust response for the final experiments. .. We obtained the following constructs from Addgene for use in our experiments: 1) for CRISPRi, Article Title: The Parkinson’s disease risk gene cathepsin B promotes fibrillar alpha-synuclein clearance, lysosomal function and glucocerebrosidase activity in dopaminergic neurons Article Snippet: .. To generate CRISPRa and CRISPRi parental cell lines, lentivirus was used to stably transduced RPE1 cells with either Plasmid Preparation:Article Title: The human Y and inactive X chromosomes similarly modulate autosomal gene expression. Article Snippet: We obtained the following constructs from Addgene for use in our experiments: 1) for CRISPRi, pLX_311-KRAB-dCas9 (Addgene: #96918), a KRAB-dCas9 blasticidin-selectable lentiviral expression vector38; 2) sgOpti (#85681), a puromycin-selectable structurally optimized guide RNA (gRNA) lentiviral expression vector67,68; 3) psPAX2 (#12260), a second generation lentiviral packaging plasmid; and 4) pCMV-VSV-G (#8454), a lentiviral envelope protein plasmid.69 We purified all plasmids using the EndoFree Maxi Kit (Qiagen). e8 Cell Genomics 4, 100462, January 10, 2024 Weused the top five guide sequences for ZFX and ZFY from the humanCRISPRi v2 gRNA library.50We tested these guides for ZFX or ZFY knockdown and chose the two guides that gave the most robust response for the final experiments. .. We obtained the following constructs from Addgene for use in our experiments: 1) for CRISPRi, Article Title: A primordial germ cell-like-cell platform enables CRISPRi screen for epigenetic fertility modifiers. Article Snippet: To construct targeting vectors containing enhancer-reporter transgenes, PCR-amplified sequences of mouse Prdm1 and Prdm14 enhancers (Murakami et al, 2016) bearing terminal NotI restriction sites were cloned into PCR4-Shh::lacZ-H11 (Addgene, #139098). .. Plasmid clones were validated by Sanger sequencing. pPyCAG-Pbase and pPB-CAGrtTA-IN (Addgene, #60612) were kindly provided by M. Azim Surani. pSpCas9(BB)-2A-PuroR (px459) V2.0 (Addgene, #62988), psPAX2 (Addgene, #12260), pMD2.G (Addgene, #12259), pLVtetO-Tfap2c (Addgene, #70269), Article Title: Tom20 gates PINK1 activity and mediates its tethering of the TOM and TIM23 translocases upon mitochondrial stress Article Snippet: .. The dCas9 plasmid used was Article Title: A primordial germ cell-like-cell platform enables CRISPRi screen for epigenetic fertility modifiers Article Snippet: To construct targeting vectors containing enhancer-reporter transgenes, PCR-amplified sequences of mouse Prdm1 and Prdm14 enhancers (Murakami et al, ) bearing terminal Not I restriction sites were cloned into PCR4- Shh::lacZ -H11 (Addgene, #139098). .. Plasmid clones were validated by Sanger sequencing. pPyCAG-Pbase and pPB-CAG-rtTA-IN (Addgene, #60612) were kindly provided by M. Azim Surani. pSpCas9(BB)-2A-PuroR (px459) V2.0 (Addgene, #62988), psPAX2 (Addgene, #12260), pMD2.G (Addgene, #12259), pLV-tetO-Tfap2c (Addgene, #70269), Purification:Article Title: The human Y and inactive X chromosomes similarly modulate autosomal gene expression. Article Snippet: We obtained the following constructs from Addgene for use in our experiments: 1) for CRISPRi, pLX_311-KRAB-dCas9 (Addgene: #96918), a KRAB-dCas9 blasticidin-selectable lentiviral expression vector38; 2) sgOpti (#85681), a puromycin-selectable structurally optimized guide RNA (gRNA) lentiviral expression vector67,68; 3) psPAX2 (#12260), a second generation lentiviral packaging plasmid; and 4) pCMV-VSV-G (#8454), a lentiviral envelope protein plasmid.69 We purified all plasmids using the EndoFree Maxi Kit (Qiagen). e8 Cell Genomics 4, 100462, January 10, 2024 Weused the top five guide sequences for ZFX and ZFY from the humanCRISPRi v2 gRNA library.50We tested these guides for ZFX or ZFY knockdown and chose the two guides that gave the most robust response for the final experiments. .. We obtained the following constructs from Addgene for use in our experiments: 1) for CRISPRi, Knockdown:Article Title: The human Y and inactive X chromosomes similarly modulate autosomal gene expression. Article Snippet: We obtained the following constructs from Addgene for use in our experiments: 1) for CRISPRi, pLX_311-KRAB-dCas9 (Addgene: #96918), a KRAB-dCas9 blasticidin-selectable lentiviral expression vector38; 2) sgOpti (#85681), a puromycin-selectable structurally optimized guide RNA (gRNA) lentiviral expression vector67,68; 3) psPAX2 (#12260), a second generation lentiviral packaging plasmid; and 4) pCMV-VSV-G (#8454), a lentiviral envelope protein plasmid.69 We purified all plasmids using the EndoFree Maxi Kit (Qiagen). e8 Cell Genomics 4, 100462, January 10, 2024 Weused the top five guide sequences for ZFX and ZFY from the humanCRISPRi v2 gRNA library.50We tested these guides for ZFX or ZFY knockdown and chose the two guides that gave the most robust response for the final experiments. .. We obtained the following constructs from Addgene for use in our experiments: 1) for CRISPRi, Stable Transfection:Article Title: The Parkinson’s disease risk gene cathepsin B promotes fibrillar alpha-synuclein clearance, lysosomal function and glucocerebrosidase activity in dopaminergic neurons Article Snippet: .. To generate CRISPRa and CRISPRi parental cell lines, lentivirus was used to stably transduced RPE1 cells with either Clone Assay:Article Title: The Parkinson’s disease risk gene cathepsin B promotes fibrillar alpha-synuclein clearance, lysosomal function and glucocerebrosidase activity in dopaminergic neurons Article Snippet: .. To generate CRISPRa and CRISPRi parental cell lines, lentivirus was used to stably transduced RPE1 cells with either Article Title: A primordial germ cell-like-cell platform enables CRISPRi screen for epigenetic fertility modifiers. Article Snippet: To construct targeting vectors containing enhancer-reporter transgenes, PCR-amplified sequences of mouse Prdm1 and Prdm14 enhancers (Murakami et al, 2016) bearing terminal NotI restriction sites were cloned into PCR4-Shh::lacZ-H11 (Addgene, #139098). .. Plasmid clones were validated by Sanger sequencing. pPyCAG-Pbase and pPB-CAGrtTA-IN (Addgene, #60612) were kindly provided by M. Azim Surani. pSpCas9(BB)-2A-PuroR (px459) V2.0 (Addgene, #62988), psPAX2 (Addgene, #12260), pMD2.G (Addgene, #12259), pLVtetO-Tfap2c (Addgene, #70269), Article Title: A primordial germ cell-like-cell platform enables CRISPRi screen for epigenetic fertility modifiers Article Snippet: To construct targeting vectors containing enhancer-reporter transgenes, PCR-amplified sequences of mouse Prdm1 and Prdm14 enhancers (Murakami et al, ) bearing terminal Not I restriction sites were cloned into PCR4- Shh::lacZ -H11 (Addgene, #139098). .. Plasmid clones were validated by Sanger sequencing. pPyCAG-Pbase and pPB-CAG-rtTA-IN (Addgene, #60612) were kindly provided by M. Azim Surani. pSpCas9(BB)-2A-PuroR (px459) V2.0 (Addgene, #62988), psPAX2 (Addgene, #12260), pMD2.G (Addgene, #12259), pLV-tetO-Tfap2c (Addgene, #70269), Sequencing:Article Title: A primordial germ cell-like-cell platform enables CRISPRi screen for epigenetic fertility modifiers. Article Snippet: To construct targeting vectors containing enhancer-reporter transgenes, PCR-amplified sequences of mouse Prdm1 and Prdm14 enhancers (Murakami et al, 2016) bearing terminal NotI restriction sites were cloned into PCR4-Shh::lacZ-H11 (Addgene, #139098). .. Plasmid clones were validated by Sanger sequencing. pPyCAG-Pbase and pPB-CAGrtTA-IN (Addgene, #60612) were kindly provided by M. Azim Surani. pSpCas9(BB)-2A-PuroR (px459) V2.0 (Addgene, #62988), psPAX2 (Addgene, #12260), pMD2.G (Addgene, #12259), pLVtetO-Tfap2c (Addgene, #70269), Article Title: A primordial germ cell-like-cell platform enables CRISPRi screen for epigenetic fertility modifiers Article Snippet: To construct targeting vectors containing enhancer-reporter transgenes, PCR-amplified sequences of mouse Prdm1 and Prdm14 enhancers (Murakami et al, ) bearing terminal Not I restriction sites were cloned into PCR4- Shh::lacZ -H11 (Addgene, #139098). .. Plasmid clones were validated by Sanger sequencing. pPyCAG-Pbase and pPB-CAG-rtTA-IN (Addgene, #60612) were kindly provided by M. Azim Surani. pSpCas9(BB)-2A-PuroR (px459) V2.0 (Addgene, #62988), psPAX2 (Addgene, #12260), pMD2.G (Addgene, #12259), pLV-tetO-Tfap2c (Addgene, #70269), Cloning:Article Title: Tom20 gates PINK1 activity and mediates its tethering of the TOM and TIM23 translocases upon mitochondrial stress Article Snippet: .. The dCas9 plasmid used was Transduction:Article Title: Energy Flux Regulates Cell Death Induced by California Serogroup Orthobunyaviruses Article Snippet: .. BE(2)-C cells were engineered to constitutively express dCas9-KRAB via lentiviral transduction of |